WB
Western blot analysis of various lysates using GRB2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.IHC
Immunohistochemistry analysis of paraffin embedded Human breast cancer using GRB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of HeLa cells using GRB2 Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). objective: 100x.IP
Immunoprecipitation analysis of 300μg extracts from 293T cells using 3μg GRB2 antibody. Western blot was performed from the immunoprecipitate using GRB2 at a dilution of 1:1000.| Product Name | GRB2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 118-217 of human GRB2 (P62993). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:6000 IHC:1:200-1:800 ICC:1:100-1:800 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GRB2 |
|---|---|
| Gene Synonyms | ASH Grb3-3 MST084 NCKAP2 MSTP084 EGFRBP-GRB2 |
| Gene Full Name | growth factor receptor bound protein 2 |
| Gene Summary | The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | ASH Grb3-3 MST084 NCKAP2 MSTP084 EGFRBP-GRB2 GRB2 |
| Molecular Weight(MW) | 25kDa |
| Cellular Localization | Cytoplasm, Endosome, Golgi apparatus, Nucleus. |
WB
Western blot analysis of various lysates using GRB2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
IHC
Immunohistochemistry analysis of paraffin embedded Human breast cancer using GRB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of HeLa cells using GRB2 Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). objective: 100x.
IP
Immunoprecipitation analysis of 300μg extracts from 293T cells using 3μg GRB2 antibody. Western blot was performed from the immunoprecipitate using GRB2 at a dilution of 1:1000.| Application Notes | WB:1:1000-1:6000 IHC:1:200-1:800 ICC:1:100-1:800 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
