Immunofluorescence
Sample Type :MCF7 cells Primary Antibody Dilution : 1:200 Secondary Antibody:Anti-rabbit-FITC Secondary Antibody Dilution: 1:500 Color/Signal Descriptions:DAPI: Blue HNRNPH1: Green Gene Name:HNRPH1 Submitted by:AnonymousImmunohistochemical
Paraffin embedded testis tissue, tested with an antibody Dilution of 5 ug/ml.Western blot
Lanes :Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate Primary Antibody Dilution : 1:4000 Secondary Antibody :Anti-rabbit-HRP Secondary Antibody Dilution : 1:5000 Gene Name :HNRPH1 Submitted by :AnonymousWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-HNRPH1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686A15170, HNRPH, hnRNPH, HNRPH1 |
| Immunogen | The immunogen for anti-HNRPH1 antibody: synthetic peptide directed towards the middle region of human HNRPH1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNRPH1 |
|---|---|
| Gene Synonyms | DKFZp686A15170 HNRPH hnRNPH HNRPH1 |
| Gene Full Name | Heterogeneous nuclear ribonucleoprotein H1 (H) |
| Gene Summary | HNRPH1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. This protein has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | DKFZp686A15170 HNRPH hnRNPH HNRPH1 |
| Molecular Weight(MW) | 49kDa |
| Sequence | 449 amino acids |
Immunofluorescence
Sample Type :MCF7 cells Primary Antibody Dilution : 1:200 Secondary Antibody:Anti-rabbit-FITC Secondary Antibody Dilution: 1:500 Color/Signal Descriptions:DAPI: Blue HNRNPH1: Green Gene Name:HNRPH1 Submitted by:Anonymous
Immunohistochemical
Paraffin embedded testis tissue, tested with an antibody Dilution of 5 ug/ml.
Western blot
Lanes :Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate Primary Antibody Dilution : 1:4000 Secondary Antibody :Anti-rabbit-HRP Secondary Antibody Dilution : 1:5000 Gene Name :HNRPH1 Submitted by :Anonymous
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
