WB
Western blot analysis of anti-HNRNPH1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human LNCAP whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human RT4 whole cell lysates, Lane 8: rat C6 whole cell lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HNRNPH1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
Flow Cytometry analysis of 293T cells using anti-HNRNPH1 antibody. ICC/IF analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in an immunocytochemical section of MCF-7 cells. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody.IF-P
IF analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in a paraffin-embedded section of human breast cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in a paraffin-embedded section of rat brain tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of 293T cells using anti-HNRNPH1 antibody. Overlay histogram showing 293T cells stained with anti-HNRNPH1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG(1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.FC
Flow Cytometry analysis of Neuro2a cells using anti-HNRNPH1 antibody. Overlay histogram showing Neuro2a cells stained with anti-HNRNPH1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH1 Antibody. Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.FC
Flow Cytometry analysis of C6 cells using anti-HNRNPH1 antibody. Overlay histogram showing C6 cells stained with anti-HNRNPH1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Anti-HNRNPH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human HnRNP H, identical to the related mouse and rat sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:1000-5000 IHC:1:50-400 ICC/IF:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNRNPH1 |
|---|---|
| Gene Synonyms | HNRPH HNRPH1 NEDCDS hnRNPH |
| Gene Full Name | heterogeneous nuclear ribonucleoprotein H1 |
| Gene Summary | This gene encodes a member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some may shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNA and is very similar to the family member HNRPF. This gene may be associated with hereditary lymphedema type I. Alternatively spliced transcript variants have been described [provided by RefSeq, Mar 2012] |
| Alternative Names | hnRNP H hnRNPH HNRNPH1 HNRPH HNRPH1 |
| Molecular Weight(MW) | 49kDa(Observed MW 49kDa) |
| Cellular Localization | Nucleus, nucleoplasm |
WB
Western blot analysis of anti-HNRNPH1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human LNCAP whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human RT4 whole cell lysates, Lane 8: rat C6 whole cell lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HNRNPH1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
Flow Cytometry analysis of 293T cells using anti-HNRNPH1 antibody. ICC/IF analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in an immunocytochemical section of MCF-7 cells. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody.
IF-P
IF analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in a paraffin-embedded section of human breast cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of HNRNPH1 using anti-HNRNPH1 antibody. HNRNPH1 was detected in a paraffin-embedded section of rat brain tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of 293T cells using anti-HNRNPH1 antibody. Overlay histogram showing 293T cells stained with anti-HNRNPH1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG(1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
FC
Flow Cytometry analysis of Neuro2a cells using anti-HNRNPH1 antibody. Overlay histogram showing Neuro2a cells stained with anti-HNRNPH1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH1 Antibody. Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
FC
Flow Cytometry analysis of C6 cells using anti-HNRNPH1 antibody. Overlay histogram showing C6 cells stained with anti-HNRNPH1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:1000-5000 IHC:1:50-400 ICC/IF:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
