Application
Antibody used in WB on Human Jurkat 1 ug/ml.Application
Antibody used in WB on Human 721_B cells at 1 ug/ml.Application
Antibody used in WB on Human MCF7 at 1 ug/ml.Application
Antibody used in IP on K562 at 1:4000 (Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPH1 ppt. k562 sample ).Application
Antibody used in IF on MCF7 cells at 1:200.Application
Antibody used in WB on HeLa S3, MCF7, K562 at 1:4000 (Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate ).Application
Antibody used in IHC on Human Testis at of 5 ug/ml.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | HNRPH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNRPH1, DKFZp686A15170, HNRPH, hnRNPH, HNRPH1 |
| Product description | HNRPH1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. This protein has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Olsen, J.V., (2006) Cell 127 (3), 635-648. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HNRPH1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HNRPH1 antibody can be used for detection of HNRPH1 by ELISA at 1:62500. HNRPH1 antibody can be used for detection of HNRPH1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNRNPH1 |
|---|---|
| Alternative Names | HNRPH1 DKFZp686A15170 HNRPH hnRNPH HNRPH1 |
| Molecular Weight(MW) | 49 kDa |
Application
Antibody used in WB on Human Jurkat 1 ug/ml.
Application
Antibody used in WB on Human 721_B cells at 1 ug/ml.
Application
Antibody used in WB on Human MCF7 at 1 ug/ml.
Application
Antibody used in IP on K562 at 1:4000 (Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPH1 ppt. k562 sample ).
Application
Antibody used in IF on MCF7 cells at 1:200.
Application
Antibody used in WB on HeLa S3, MCF7, K562 at 1:4000 (Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate ).
Application
Antibody used in IHC on Human Testis at of 5 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | HNRPH1 antibody can be used for detection of HNRPH1 by ELISA at 1:62500. HNRPH1 antibody can be used for detection of HNRPH1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HNRPH1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
