Western blot
Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate Primary Antibody Dilution : 1:1000Secondary Antibody : Goat anti-rabbit-HRP Secondry Antibody Dilution : 1:5000Submitted by: Jose Luis Rosa, Universitat de BarcelonaWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-PPP1CA polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC15877, MGC1674, PP-1A, PPP1A, PP1alpha |
| Immunogen | The immunogen for anti-PPP1CA antibody: synthetic peptide directed towards the N terminal of human PPP1CA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMousePig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPP1CA |
|---|---|
| Gene Synonyms | MGC15877 MGC1674 PP-1A PPP1A PP1alpha |
| Gene Full Name | Protein phosphatase 1, catalytic subunit, alpha isozyme |
| Gene Summary | PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory.The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | MGC15877 MGC1674 PP-1A PPP1A PP1alpha |
| Molecular Weight(MW) | 38kDa |
| Sequence | 341 amino acids |
| Entrez Gene | 5499 |
|---|---|
| SwissProt ID | Q07161 |
| Protein Accession | NP_001008709 |
Western blot
Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate Primary Antibody Dilution : 1:1000Secondary Antibody : Goat anti-rabbit-HRP Secondry Antibody Dilution : 1:5000Submitted by: Jose Luis Rosa, Universitat de Barcelona
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
