Western blot
Sample Type: 1. Human NT-2 cells (60ug) 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human kidney| Product Name | Rabbit anti-ISG15 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G1P2, IFI15, UCRP, IP17, hUCRP |
| Immunogen | The immunogen for anti-ISG15 antibody: synthetic peptide directed towards the middle region of human ISG15 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanPigRabbitSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ISG15 |
|---|---|
| Gene Synonyms | G1P2 IFI15 UCRP IP17 hUCRP |
| Gene Full Name | ISG15 ubiquitin-like modifier |
| Gene Summary | ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha. |
| Alternative Names | G1P2 IFI15 UCRP IP17 hUCRP |
| Molecular Weight(MW) | 17kDa |
| Sequence | 165 amino acids |
Western blot
Sample Type: 1. Human NT-2 cells (60ug) 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human kidney| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
