WB
Western blot analysis of ISG15 on different lysates with Rabbit anti-ISG15 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.| Product Name | ISG15 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant full length protein of Human ISG15. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ISG15 |
|---|---|
| Gene Synonyms | G1P2 IP17 UCRP IFI15 IMD38 hUCRP |
| Gene Full Name | ISG15 ubiquitin like modifier |
| Gene Summary | The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012] |
| Alternative Names | G1P2 antibody hUCRP antibody IFI 15 antibody IFI15 antibody Interferon alpha inducible protein antibody Interferon induced protein 15 antibody Interferon induced protein IFI-15K antibody Interferon stimulated protein 15 kDa antibody Interferon alpha-inducible protein (clone IFI-15K) antibody Interferon-induced 15 kDa protein antibody Interferon-induced 17 kDa protein antibody Interferon-induced 17-kDa/15-kDa protein antibody IP17 antibody ISG 15 antibody ISG15 antibody ISG15 ubiquitin like modifier antibody ISG15_HUMAN antibody Ubiquitin cross-reactive protein antibody Ubiquitin-like protein ISG15 antibody UCRP antibody |
| Molecular Weight(MW) | 18 kDa(Observed band size: 14 kDa) |
| Cellular Localization | Cytoplasm, Secreted. |
WB
Western blot analysis of ISG15 on different lysates with Rabbit anti-ISG15 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
