WB
Untreated and IFN alpha treated A459 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with ISG15 antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with ISG15 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with ISG15 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using ISG15 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using ISG15 Antibody at dilution of 1:50 (under 10x lens).ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using ISG15 antibody at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | ISG15 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ISG15 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ISG15 |
|---|---|
| Gene Synonyms | G1P2 IP17 UCRP IFI15 IMD38 hUCRP |
| Gene Full Name | ISG15 ubiquitin like modifier |
| Gene Summary | The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012] |
| Alternative Names | G1P2 hUCRP IFI15 Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein |
| Molecular Weight(MW) | 18 kDa(Observed band size:15-17 kDa) |
| Cellular Localization | Cytoplasm,Secreted |
WB
Untreated and IFN alpha treated A459 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with ISG15 antibody at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with ISG15 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with ISG15 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using ISG15 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using ISG15 Antibody at dilution of 1:50 (under 10x lens).
ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using ISG15 antibody at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
