WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ISG15 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela, Lane 2: PC-3.IHC
Immunofluorescence analysis of HEK293. Picture A: ISG15 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B| Product Name | ISG15 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ISG15 |
|---|---|
| Gene Synonyms | G1P2 IP17 UCRP IFI15 IMD38 hUCRP |
| Gene Full Name | ISG15 ubiquitin like modifier |
| Gene Summary | The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012] |
| Molecular Weight(MW) | 18kD |
| Cellular Localization | Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ISG15 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela, Lane 2: PC-3.
IHC
Immunofluorescence analysis of HEK293. Picture A: ISG15 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B| Application Notes | WB:1:500-1:2000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
