Immunofluorescence
Sample Type: outer mouse plexiform layerRed: PrimaryBlue: DAPIPrimary Dilution: 1:200Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L)Secondary Dilution: 1:200Image Submitted by: David ZenisekYale UniversityImmunofluorescence
Sample Type: complete mouse retina sectionsRed: PrimaryBlue: DAPIPrimary Dilution: 1:200Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L)Secondary Dilution: 1:200Image Submitted by: David ZenisekYale UniversityImmunoblot
Lane1: 10ug mouse cortex brain lysateLane2: 25ug mouse cortex brain lysateLane3: 40ug mouse cortex brain lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: Syt1Submitted by: AnonymousWestern blot
Syt1 antibody - middle region validated by WB using Mouse Spleen lysate at 1.0ug/ml.| Product Name | Rabbit anti-Syt1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AW124717, G630098F17Rik, SytI |
| Immunogen | The immunogen for anti-Syt1 antibody: synthetic peptide corresponding to a region of Mouse |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Syt1 |
|---|---|
| Gene Synonyms | AW124717 G630098F17Rik SytI |
| Gene Full Name | Synaptotagmin I |
| Gene Summary | Syt1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2. |
| Alternative Names | AW124717 G630098F17Rik SytI |
| Molecular Weight(MW) | 47kDa |
| Sequence | 421 amino acids |
Immunofluorescence
Sample Type: outer mouse plexiform layerRed: PrimaryBlue: DAPIPrimary Dilution: 1:200Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L)Secondary Dilution: 1:200Image Submitted by: David ZenisekYale University
Immunofluorescence
Sample Type: complete mouse retina sectionsRed: PrimaryBlue: DAPIPrimary Dilution: 1:200Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L)Secondary Dilution: 1:200Image Submitted by: David ZenisekYale University
Immunoblot
Lane1: 10ug mouse cortex brain lysateLane2: 25ug mouse cortex brain lysateLane3: 40ug mouse cortex brain lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: Syt1Submitted by: Anonymous
Western blot
Syt1 antibody - middle region validated by WB using Mouse Spleen lysate at 1.0ug/ml.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
