Application
Western blot of rat cortex lysate showing specific labeling of the ~65k synaptotagmin protein.| Product Name | Synaptotagmin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P65, Synaptotagmin I, SytI |
| Product description | Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006).1) Littleton JT, Bellen HJ (1995) Synaptotagmin controls and modulates synaptic vesicle fusion in a Ca(2+) dependent manner. Trends Neurosci. Apr;18(4):177-83. |
| Immunogen | Synthetic peptide corresponding to amino acid residues within the synaptotagmin I protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineChickenHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Syt1 |
|---|---|
| Alternative Names | P65 Synaptotagmin I SytI |
| Molecular Weight(MW) | 65 |
Application
Western blot of rat cortex lysate showing specific labeling of the ~65k synaptotagmin protein.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Synaptotagmin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
