Western blot
Positive Control: Lane 1: 80ug pig serum Primary Antibody Dilution : 1:1000Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:1000Submitted by: Martina Ondrovics, University of Veterinary Medicine ViennaWestern blot
Titration: 1.0 ug/mlPositive Control: Mouse HeartImmunoblot
Lane1: 10ug mouse cortex brain lysateLane2: 25ug mouse cortex brain lysateLane3: 40ug mouse cortex brain lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: Hspa1aSubmitted by: Anonymous| Product Name | Rabbit anti-Hspa1a polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hsp70-3, Hsp70.3, Hsp72, MGC189852, hsp68, hsp70A1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Hspa1a |
|---|---|
| Gene Synonyms | Hsp70-3 Hsp70.3 Hsp72 MGC189852 hsp68 hsp70A1 |
| Gene Full Name | Heat shock protein 1A |
| Gene Summary | In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. |
| Alternative Names | Hsp70-3 Hsp70.3 Hsp72 MGC189852 hsp68 hsp70A1 |
| Molecular Weight(MW) | 70kDa |
| Sequence | 641 amino acids |
Western blot
Positive Control: Lane 1: 80ug pig serum Primary Antibody Dilution : 1:1000Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:1000Submitted by: Martina Ondrovics, University of Veterinary Medicine Vienna
Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Heart
Immunoblot
Lane1: 10ug mouse cortex brain lysateLane2: 25ug mouse cortex brain lysateLane3: 40ug mouse cortex brain lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: Hspa1aSubmitted by: Anonymous| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM118642