Western blot
Titration: 1.0 ug/mlPositive Control: Hela Whole Cell| Product Name | Rabbit anti-EIF3A polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF3, EIF3S10, KIAA0139, P167, TIF32, eIF3-p170, eIF3-theta, p180, p185 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF3A |
|---|---|
| Gene Synonyms | EIF3 EIF3S10 KIAA0139 P167 TIF32 eIF3-p170 eIF3-theta p180 p185 |
| Gene Full Name | Eukaryotic translation initiation factor 3, subunit A |
| Gene Summary | EIF3A is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. |
| Alternative Names | EIF3 EIF3S10 KIAA0139 P167 TIF32 eIF3-p170 eIF3-theta p180 p185 |
| Molecular Weight(MW) | 152kDa |
| Sequence | 1382 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: Hela Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
