Application
ITGB4BP Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ITGB4BP antibody detected the ITGB4BP protein (arrow).Application
ITGB4BP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITGB4BP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ITGB4BP Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF6; EIF3A; ITGB4BP; Eukaryotic translation initiation factor 6; B(2)GCN homolog; B4 integrin interactor; CAB; p27(BBP) |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIF3A ITGB4BP |
|---|---|
| Alternative Names | EIF6 EIF3A ITGB4BP Eukaryotic translation initiation factor 6 B(2)GCN homolog B4 integrin interactor CAB p27(BBP) |
| Molecular Weight(MW) | 26599 Da |
| Function | Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity |
| Tissue Specificity | Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level) |
| Cellular Localization | Cytoplasm. Nucleus, nucleolus. Note=Shuttles between cytoplasm and nucleus/nucleolus |
Application
ITGB4BP Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ITGB4BP antibody detected the ITGB4BP protein (arrow).
Application
ITGB4BP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITGB4BP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
