Application
Western blot analysis of ITGB4BP Antibody (Center) in MDA-MB231, Hela cell line lysates (35ug/lane). ITGB4BP (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ITGB4BP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of widr cells using ITGB4BP Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ITGB4BP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF6; EIF3A; ITGB4BP; Eukaryotic translation initiation factor 6; B(2)GCN homolog; B4 integrin interactor; CAB; p27(BBP) |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIF3A ITGB4BP |
|---|---|
| Alternative Names | EIF6 EIF3A ITGB4BP Eukaryotic translation initiation factor 6 B(2)GCN homolog B4 integrin interactor CAB p27(BBP) |
| Molecular Weight(MW) | 26599 Da |
| Function | Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity |
| Tissue Specificity | Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level) |
| Cellular Localization | Cytoplasm. Nucleus, nucleolus. Note=Shuttles between cytoplasm and nucleus/nucleolus |
| Entrez Gene | 3692 |
|---|
Application
Western blot analysis of ITGB4BP Antibody (Center) in MDA-MB231, Hela cell line lysates (35ug/lane). ITGB4BP (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ITGB4BP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of widr cells using ITGB4BP Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
