Western blot
Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : Anti rabbit-HRP Secondry Antibody Dilution : 1:5,000Submitted by: Scott Wilson, University of Alabama at BirminghamWestern blot
Titration: 1.0 ug/mlPositive Control: Hela Whole Cell| Product Name | Rabbit anti-STAM polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686J2352, STAM1, STAM-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAM |
|---|---|
| Gene Synonyms | DKFZp686J2352 STAM1 STAM-1 |
| Gene Full Name | Signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 |
| Gene Summary | This gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The encoded protein also associates with hepatocyte growth factor-regulated substrate to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for this gene. |
| Alternative Names | DKFZp686J2352 STAM1 STAM-1 |
| Molecular Weight(MW) | 59kDa |
| Sequence | 540 amino acids |
Western blot
Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : Anti rabbit-HRP Secondry Antibody Dilution : 1:5,000Submitted by: Scott Wilson, University of Alabama at Birmingham
Western blot
Titration: 1.0 ug/mlPositive Control: Hela Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
