Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-STAM Antibody in Hela cell line lysates (35ug/lane)Application
Western blot analysis of STAM using rabbit polyclonal STAM Antibody (P341) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STAM gene.| Product Name | STAM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Signal transducing adapter molecule 1, STAM-1, STAM, STAM1 |
| Product description | Stimulation of cells with cytokines initiates a signal transduction cascade involving cytokine receptors, Janus kinases (JAKs) and signal transducers and activators of transcription (STATs). STAM for 'signal-transducing adaptor molecule, induced after stimulation of cells with cytokine IL2, is a component of signal transduction downstream of JAK3.1 Human STAM cDNA cloned from a T-cell cDNA library encodes a 540-amino acid protein precipitated by anti-phosphotyrosine. Northern blot analysis indicates that STAM is expressed as a 2.9-kb message in a wide variety of tissue and cell types. The STAM sequence contains a Src-homology 3 (SH3) domain and an immunoreceptor tyrosine-based activation motif (ITAM). It has been suggested that STAM acts as an adaptor molecule in signal transduction pathways from cytokine receptors. |
| Immunogen | This STAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-356 amino acids from the C-terminal region of human STAM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | STAM |
|---|---|
| Alternative Names | Signal transducing adapter molecule 1 STAM-1 STAM STAM1 |
| Molecular Weight(MW) | 59 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-STAM Antibody in Hela cell line lysates (35ug/lane)
Application
Western blot analysis of STAM using rabbit polyclonal STAM Antibody (P341) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STAM gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
