Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Product Name | Rabbit anti-SPAM1 Antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HYA1, HYAL1, HYAL3, HYAL5, MGC26532, PH-20, PH20, SPAG15 |
| Immunogen | The immunogen for Anti-SPAM1 antibody is: synthetic peptide directed towards the C-terminal region of Human SPAM1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPAM1 |
|---|---|
| Gene Synonyms | HYA1 HYAL1 HYAL3 HYAL5 MGC26532 PH-20 PH20 SPAG15 |
| Gene Summary | Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | HYA1 HYAL1 HYAL3 HYAL5 MGC26532 PH-20 PH20 SPAG15 |
| Molecular Weight(MW) | 58kDa |
| Sequence | 509 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
