Immunofluorescence
Immunofluorescence analysis of HuvEc cells, using COT (Phospho-Thr290) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from 293 cells treated with UV 15', using COT (Phospho-Thr290) Antibody. The lane on the right is treated with the synthesized peptide| Product Name | Rabbit anti-COT (Phospho-Thr290) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human COT around the phosphorylation site of Thr290. |
| Modification | p-Thr290 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBIHCIFELISA |
| WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:40000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K8 |
|---|---|
| Gene Synonyms | C-COT COT proto-oncogene serine/threonine-protein kinase Cancer Osaka thyroid oncogene M3K8 MAP3K8 Mitogen-activated protein kinase kinase kinase 8 TPL-2 TPL2 Tpl2 Tumor progression locus 2 kinase Cot |
Immunofluorescence
Immunofluorescence analysis of HuvEc cells, using COT (Phospho-Thr290) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from 293 cells treated with UV 15', using COT (Phospho-Thr290) Antibody. The lane on the right is treated with the synthesized peptide| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:40000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
