Application
Figure 1: Western blot analysis using anti-LSD1 monoclonal antiobdy against COS (1), Hela (2), NIH/3T3(3), A549 (4) and Jurkat (5) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma(left) and kidney carcinoma(right), showing nuclear localization using anti-LSD1 antibody with DAB staining.| Product Name | LSD1/AOF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KDM1; LSD1; AOF2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1B2E5 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AOF2 KDM1 KIAA0601 LSD1 |
|---|---|
| Alternative Names | KDM1 LSD1 AOF2 |
| Molecular Weight(MW) | 92903 Da |
| Function | Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development Effector of SNAI1-mediated transcription repression of E- cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 23028 |
|---|---|
| Nucleotide Accession | NP_055828.2 |
Application
Figure 1: Western blot analysis using anti-LSD1 monoclonal antiobdy against COS (1), Hela (2), NIH/3T3(3), A549 (4) and Jurkat (5) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma(left) and kidney carcinoma(right), showing nuclear localization using anti-LSD1 antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human LSD1 expressed in E. Coli. |
