Application
MAD1L1 Antibody (N-term) (Cat. #AP12347a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MAD1L1 antibody detected the MAD1L1 protein (arrow).| Product Name | MAD1L1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAD1L1; MAD1; TXBP181; Mitotic spindle assembly checkpoint protein MAD1; Mitotic arrest deficient 1-like protein 1; Mitotic checkpoint MAD1 protein homolog; Tax-binding protein 181 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAD1 TXBP181 |
|---|---|
| Alternative Names | MAD1L1 MAD1 TXBP181 Mitotic spindle assembly checkpoint protein MAD1 Mitotic arrest deficient 1-like protein 1 Mitotic checkpoint MAD1 protein homolog Tax-binding protein 181 |
| Molecular Weight(MW) | 83067 Da |
| Function | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding |
| Tissue Specificity | Expressed weakly at G0/G1 and highly at late S and G2/M phase |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle. Note=From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Co-localizes with NEK2 at the kinetochore |
| Entrez Gene | 8379 |
|---|---|
| Nucleotide Accession | NP_001013859.1 NP_001013858.1 NP_003541.2 |
Application
MAD1L1 Antibody (N-term) (Cat. #AP12347a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MAD1L1 antibody detected the MAD1L1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
