WB
Western blot analysis of MAD1L1 on different lysates with Rabbit anti-MAD1L1 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Product Name | MAD1L1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human MAD1L1 aa 31-180 / 718. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAD1L1 |
|---|---|
| Gene Synonyms | MAD1 MVA7 PIG9 TP53I9 TXBP181 |
| Gene Full Name | mitotic arrest deficient 1 like 1 |
| Gene Summary | MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
| Alternative Names | hMAD1 antibody HsMAD1 antibody MAD1 antibody MAD1 mitotic arrest deficient like 1 (yeast) antibody MAD1-like protein 1 antibody MAD1L1 antibody MD1L1_HUMAN antibody Mitotic arrest deficient 1 antibody Mitotic arrest deficient 1-like protein 1 antibody Mitotic checkpoint MAD1 protein homolog antibody Mitotic spindle assembly checkpoint protein MAD1 antibody PIG9 antibody Tax binding protein 181 antibody Tax-binding protein 181 antibody TP53I9 antibody Tumor protein p53 inducible protein 9 antibody TXBP181 antibody |
| Molecular Weight(MW) | 83 kDa |
| Cellular Localization | Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus. |
WB
Western blot analysis of MAD1L1 on different lysates with Rabbit anti-MAD1L1 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
