Application
MAD2L1 Antibody (Center) (Cat. #AP13837c) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MAD2L1 antibody detected the MAD2L1 protein (arrow).Application
MAD2L1 Antibody (Center) (Cat. #AP13837c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAD2L1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | MAD2L1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAD2L1; MAD2; Mitotic spindle assembly checkpoint protein MAD2A; Mitotic arrest deficient 2-like protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAD2 |
|---|---|
| Alternative Names | MAD2L1 MAD2 Mitotic spindle assembly checkpoint protein MAD2A Mitotic arrest deficient 2-like protein 1 |
| Molecular Weight(MW) | 23510 Da |
| Function | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore- spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate |
| Tissue Specificity | This MAD2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-121 amino acids from the Central region of human MAD2L1. |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole Note=Recruited by MAD1L1 to unattached kinetochores (Probable) Recruited to the nuclear pore complex by TPR during interphase Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD |
| Entrez Gene | 4085 |
|---|---|
| Nucleotide Accession | NP_002349.1 |
Application
MAD2L1 Antibody (Center) (Cat. #AP13837c) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MAD2L1 antibody detected the MAD2L1 protein (arrow).
Application
MAD2L1 Antibody (Center) (Cat. #AP13837c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAD2L1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
