Immunohistochemistry
IHC Suggested Anti-MXI1 antibody Titration: 5µg/ mlPositive Control: Lung, respiratory epitheliumWB
MT protects PTr2 cells from H2O2-induced oxidative stress by inducing proteins expression. Western blot analysis were performed to detect the protein levels in different group.| Product Name | Rabbit anti-MXI1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-MXI1 antibody: synthetic peptide directed towards the middle region of human MXI1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MXI1 |
|---|---|
| Gene Synonyms | MAD2 MGC43220 MXD2 MXI bHLHc11 |
| Gene Full Name | MAX interactor 1 |
| Gene Summary | Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The MXI1 gene encodes a transcriptional repressor protein thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in MXI1 are frequently found in patients with prostate tumors.Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally. |
| Alternative Names | MAD2 MGC43220 MXD2 MXI bHLHc11 |
| Molecular Weight(MW) | 26kDa |
| Sequence | 295 amino acids |
Immunohistochemistry
IHC Suggested Anti-MXI1 antibody Titration: 5µg/ mlPositive Control: Lung, respiratory epithelium
WB
MT protects PTr2 cells from H2O2-induced oxidative stress by inducing proteins expression. Western blot analysis were performed to detect the protein levels in different group.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
