Application
Antibody used in WB on Human SH-SYSY at 0.2-1 ug/ml.| Product Name | MXI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The MXI1 gene encodes a transcriptional repressor protein thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in MXI1 are frequently found in patients with prostate tumors. Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally.1) Johnson, K.R., (2007) FEBS J. 274 (17), 4643-4653 . |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MXI1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MXI1 antibody can be used for detection of MXI1 by ELISA at 1:62500. MXI1 antibody can be used for detection of MXI1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MXI1 |
|---|---|
| Alternative Names | MXI1 MAD2 MGC43220 MXD2 MXI bHLHc11 |
| Molecular Weight(MW) | 33 kDa |
Application
Antibody used in WB on Human SH-SYSY at 0.2-1 ug/ml.| Positive Control | 1) SH-SYSY Cell Lysate |
|---|---|
| Application Notes | MXI1 antibody can be used for detection of MXI1 by ELISA at 1:62500. MXI1 antibody can be used for detection of MXI1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MXI1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
