Application
Western blot analysis of c-Kit expression in HEL 92.1.7 (A) and TT (B) whole cell lysates.Application
FCM analysis of TF-1 cells. Black line histogram represents the isotype control, normal mouse IgG1: .Application
Immunofluorescence staining of methanol-fixed TF-1 cells showing membrane staining.Application
Western blot analysis of c-Kit expression in CCRF-HSB-2 (A) and HEL 92.1.7 (B) whole cell lysates.| Product Name | c-Kit Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The c-Kit proto-oncogene is a member of the receptor tyrosine kinase family and, more specifically, is closely related to the platelet derived growth factor receptor (PDGFR). c-Kit, the normal cellular homolog of the HZ4-feline sarcoma virus transforming gene (v-Kit), encodes a transmembrane receptor. c-Kit regulates a variety of biological responses including chemotaxis, cell proliferation, apoptosis and adhesion. c-Kit is also identical with the product of the W locus in mice and, as such, is integral to the development of mast cells and hematopoiesis. The ligand for the c-Kit receptor (KL) has been identified and is encoded at the murine steel (SI) locus. Kit is the human homolog of the proto-oncogene c-Kit. Mutations in Kit are integral for tumor growth and progression in various cancers. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of c-Kit of human origin by WB, IP, IF, FCM and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against full length c-Kit of human origin |
|---|---|
| Tissue Specificity | raised against full length c-Kit of human origin |
| Entrez Gene | 3815 |
|---|
Application
Western blot analysis of c-Kit expression in HEL 92.1.7 (A) and TT (B) whole cell lysates.
Application
FCM analysis of TF-1 cells. Black line histogram represents the isotype control, normal mouse IgG1: .
Application
Immunofluorescence staining of methanol-fixed TF-1 cells showing membrane staining.
Application
Western blot analysis of c-Kit expression in CCRF-HSB-2 (A) and HEL 92.1.7 (B) whole cell lysates.| Application Notes | recommended for detection of c-Kit of human origin by WB, IP, IF, FCM and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
