OM183980.jpg" data-gallery-id="gallery2"
data-title="WB"
data-description="Protein expression levels of e,f) F-actin and g,h) MACF1 measured by western blot: Immunoblot plots of e,g) the proteins, and f,h) ratio of the target protein to the internal reference protein (n = 3)."
data-app="WB"
alt="WB"
onerror="this.src='//www.omnimabs.com/content/xtheme/oumeini/images/noimage.jpg'">
OM183980.jpg" data-gallery-id="gallery1" loading="lazy" decoding="async" style="object-fit: contain;"
data-title="WB"
data-description="Protein expression levels of e,f) F-actin and g,h) MACF1 measured by western blot: Immunoblot plots of e,g) the proteins, and f,h) ratio of the target protein to the internal reference protein (n = 3)."
data-app="WB"
alt="WB">
OM183980.jpg" onerror="this.src='//www.omnimabs.com/content/xtheme/oumeini/images/noimage.jpg'" />
Application
Western blot analysis of MACF1 expression in mouse brain tissue extract.WB
Protein expression levels of e,f) F-actin and g,h) MACF1 measured by western blot: Immunoblot plots of e,g) the proteins, and f,h) ratio of the target protein to the internal reference protein (n = 3).| Product Name | MACF1 Antibody (M-244) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | MACF1 (Microtubule-actin cross-linking factor 1) is a 5,327 amino acid protein that is encoded by the human gene MACF1. MACF1 belongs to the plakin or cytolinker family and contains one actin-binding domain, two CH (calponin-homology) domains, two EF-hand domains, one SH3 domain and thirty-seven spectrin repeats. MACF1 is an F-actin-binding protein which may play a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules. The spectrin repeats, an important feature found in many proteins involved in cytoskeletal structure, forms a three helix bundle with the second helix (with proline interrupts in some sequences). MACF1 is a cytoplasmic protein expressed mainly in lung, brain, spinal cord, skeletal and cardiac muscle, and skin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| recommended for detection of MACF1 of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1997-2240 mapping within an internal region of MACF1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1997-2240 mapping within an internal region of MACF1 of mouse origin |
| Entrez Gene | 11426 |
|---|
Application
Western blot analysis of MACF1 expression in mouse brain tissue extract.OM183980.jpg" alt="" rel="/upload/0201/OM200318 OM183980.jpg" class="zoomify" />
WB
Protein expression levels of e,f) F-actin and g,h) MACF1 measured by western blot: Immunoblot plots of e,g) the proteins, and f,h) ratio of the target protein to the internal reference protein (n = 3).| Application Notes | recommended for detection of MACF1 of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
