Application
Figure 1: Immunohistochemical analysis of paraffin-embedded human thymoma tissue(left) and spleen tissue(right), showing cytoplasmic localization using anti-MAP2K4 antibody with DAB staining.| Product Name | MAP2K4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 2D10D8,4G11B2,2D10C4,2D10C3 |
| Host Species | Mouse |
| Tested Applications | IHC |
| IHC:1:200 - 1000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, |
|---|---|
| Alternative Names | MAP2K4 |
| Molecular Weight(MW) | 44288 Da |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14 |
| Tissue Specificity | Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity) |
| Entrez Gene | 6416 |
|---|---|
| Nucleotide Accession | NP_003001.1 |
Application
Figure 1: Immunohistochemical analysis of paraffin-embedded human thymoma tissue(left) and spleen tissue(right), showing cytoplasmic localization using anti-MAP2K4 antibody with DAB staining.| Application Notes | IHC:1:200 - 1000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of MAP2K4 expressed in E. Coli. |
