Application
MASTL (Cat. #AM1911b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MASTL antibody detected the MASTL protein (arrow).Application
Confocal immunofluorescent analysis of MASTL Antibody (Cat#AM1911b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | MASTL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MASTL; GW; GWL; THC2; Serine/threonine-protein kinase greatwall; Microtubule-associated serine/threonine-protein kinase-like |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 235CT7.8.2.3 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GW GWL THC2 |
|---|---|
| Alternative Names | MASTL GW GWL THC2 Serine/threonine-protein kinase greatwall Microtubule-associated serine/threonine-protein kinase-like |
| Molecular Weight(MW) | 97319 Da |
| Function | Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation |
| Tissue Specificity | This MASTL monoclonal antibody is generated from mouse immunized with MASTL recombinant protein. |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Nucleus. Cleavage furrow. Note=During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow |
| Entrez Gene | 84930 |
|---|---|
| Nucleotide Accession | NP_001165774.1 |
Application
MASTL (Cat. #AM1911b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MASTL antibody detected the MASTL protein (arrow).
Application
Confocal immunofluorescent analysis of MASTL Antibody (Cat#AM1911b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
