Application
Western blot analysis of MCC over-expressed 293 cell line, cotransfected with MCC Validated Chimera RNAi ( Cat # H00004163-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCC monoclonal antibody (M01), clone 1A7 (Cat # AT2815a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Application
Western Blot analysis of MCC expression in transfected 293T cell line by MCC monoclonal antibody (M01), clone 1A7.Lane 1: MCC transfected lysate(93.1 KDa).Lane 2: Non-transfected lysate.| Product Name | MCC Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ46755mutated in colorectal cancers |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1A7 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | FLJ46755mutated in colorectal cancers |
|---|---|
| Molecular Weight(MW) | 93027 Da |
| Tissue Specificity | MCC (AAH09279, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Entrez Gene | 4163 |
|---|
Application
Western blot analysis of MCC over-expressed 293 cell line, cotransfected with MCC Validated Chimera RNAi ( Cat # H00004163-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCC monoclonal antibody (M01), clone 1A7 (Cat # AT2815a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Application
Western Blot analysis of MCC expression in transfected 293T cell line by MCC monoclonal antibody (M01), clone 1A7.Lane 1: MCC transfected lysate(93.1 KDa).Lane 2: Non-transfected lysate.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
