Application
A. Western blot analysis on (A)HeLa and (B) MCF-7 cell lysate using anti-MCM5 RabMAb (Cat. #AJ1464a), dilution 1:10,000.Application
B. Immunohistochemical analysis of paraffin-embedded human squamous cell cervical carcinoma using anti-MCM5 RabMAb. (Cat. #AJ1464a)Application
C. Immunofluorescent staining of HeLa cells using anti-MCM5 RabMAb (Cat. #AJ1464a).| Product Name | MCM5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCM5, CDC46, DNA replication licensing factor MCM5, CDC46 homolog;P1-CDC46 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2683Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDC46 |
|---|---|
| Alternative Names | MCM5 CDC46 DNA replication licensing factor MCM5 CDC46 homolog P1-CDC46 |
| Molecular Weight(MW) | 82286 Da |
| Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP |
| Tissue Specificity | A synthetic peptide corresponding to residues near the C-term of human MCM5 was used as an immunogen. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4174 |
|---|
Application
A. Western blot analysis on (A)HeLa and (B) MCF-7 cell lysate using anti-MCM5 RabMAb (Cat. #AJ1464a), dilution 1:10,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human squamous cell cervical carcinoma using anti-MCM5 RabMAb. (Cat. #AJ1464a)
Application
C. Immunofluorescent staining of HeLa cells using anti-MCM5 RabMAb (Cat. #AJ1464a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
