Application
Western blot analysis of MCM5 Antibody (C-term) pre-incubated without(lane 1) and with(lane 2) blocking peptide in NCI-H460 cell line lysate. MCM5 (arrow) was detected using the purified Pab (1:60 dilution).Application
MCM5 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MCM5 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MCM5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCM5; CDC46; DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDC46 |
|---|---|
| Alternative Names | MCM5 CDC46 DNA replication licensing factor MCM5 CDC46 homolog P1-CDC46 |
| Molecular Weight(MW) | 82286 Da |
| Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP |
| Tissue Specificity | This MCM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705~734 amino acids from the C-terminal region of human MCM5. |
| Cellular Localization | Nucleus. |
Application
Western blot analysis of MCM5 Antibody (C-term) pre-incubated without(lane 1) and with(lane 2) blocking peptide in NCI-H460 cell line lysate. MCM5 (arrow) was detected using the purified Pab (1:60 dilution).
Application
MCM5 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MCM5 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
