Application
MCM5 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the MCM5 antibody detected the MCM5 protein (arrow).Application
MCM5 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MCM5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCM5; CDC46; DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDC46 |
|---|---|
| Alternative Names | MCM5 CDC46 DNA replication licensing factor MCM5 CDC46 homolog P1-CDC46 |
| Molecular Weight(MW) | 82286 Da |
| Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP |
| Tissue Specificity | This MCM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-75 amino acids from the N-terminal region of human MCM5. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4174 |
|---|---|
| Nucleotide Accession | NP_006730.2 |
Application
MCM5 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the MCM5 antibody detected the MCM5 protein (arrow).
Application
MCM5 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
