Application
Western blot analysis of anti-MEKK1 Pab in HL-60 cell lysate. MEKK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Product Name | MEKK1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP3K1; MAPKKK1; MEKK; MEKK1; Mitogen-activated protein kinase kinase kinase 1; MAPK/ERK kinase kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAPKKK1 MEKK MEKK1 |
|---|---|
| Alternative Names | MAP3K1 MAPKKK1 MEKK MEKK1 Mitogen-activated protein kinase kinase kinase 1 MAPK/ERK kinase kinase 1 |
| Molecular Weight(MW) | 164470 Da |
| Function | Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway |
| Tissue Specificity | This MEKK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1136~1166 amino acids from the C-terminal region of human MEKK1. |
| Entrez Gene | 4214 |
|---|
Application
Western blot analysis of anti-MEKK1 Pab in HL-60 cell lysate. MEKK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
