Application
Western blot analysis of anti-MEN1 Antibody (T594) in Ramos cell line lysates (35ug/lane). MEN1(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MEN1 Antibody (T594) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of MEN1 Antibody (T594)(Cat#AP7415b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | MEN1 Antibody (T594) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MEN1; SCG2; Menin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SCG2 |
|---|---|
| Alternative Names | MEN1 SCG2 Menin |
| Molecular Weight(MW) | 68023 Da |
| Function | Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus. Note=Concentrated in nuclear body- like structures. Relocates to the nuclear matrix upon gamma irradiation |
Application
Western blot analysis of anti-MEN1 Antibody (T594) in Ramos cell line lysates (35ug/lane). MEN1(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MEN1 Antibody (T594) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of MEN1 Antibody (T594)(Cat#AP7415b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
