Application
A. Western blot analysis on Hek 293 cell lysate using anti-MerTK RabMAb (Cat. #AJ1474a), dilution 1:1,000.Application
B. Immunohistochemical analysis of paraffin-embedded human lymphoma using anti-MerTK RabMAb (Cat. #AJ1474a).| Product Name | MerTK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MERTK, MER, Proto-oncogene tyrosine-protein kinase MER, Receptor tyrosine kinase MerTK |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y323 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MER |
|---|---|
| Alternative Names | MERTK MER Proto-oncogene tyrosine-protein kinase MER Receptor tyrosine kinase MerTK |
| Molecular Weight(MW) | 110249 Da |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll- like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3 |
| Tissue Specificity | Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver |
| Cellular Localization | Membrane; Single-pass type I membrane protein (By similarity) |
| Entrez Gene | 10461 |
|---|
Application
A. Western blot analysis on Hek 293 cell lysate using anti-MerTK RabMAb (Cat. #AJ1474a), dilution 1:1,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human lymphoma using anti-MerTK RabMAb (Cat. #AJ1474a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
