Application
Figure 1: Western blot analysis using anti-Metadherin mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human liver cancer(A) and brain tissue(B), showing cytoplasmic localization using anti-Metadherin antibody with DAB staining.Application
Figure 3: Flow cytometric analysis of Hela cells using anti-Metadherin mAb (green) and negative control (purple).| Product Name | Metadherin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 3D3; AEG1; LYRIC; MTDH |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2F11C3 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AEG1 LYRIC |
|---|---|
| Alternative Names | 3D3 AEG1 LYRIC MTDH |
| Molecular Weight(MW) | 63837 Da |
| Function | Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF- kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance |
| Tissue Specificity | Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein (By similarity). Cell junction, tight junction (By similarity) Nucleus, nucleolus (By similarity). Cytoplasm, perinuclear region Note=In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus |
| Entrez Gene | 92140 |
|---|---|
| Nucleotide Accession | NP_848927.2 |
Application
Figure 1: Western blot analysis using anti-Metadherin mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human liver cancer(A) and brain tissue(B), showing cytoplasmic localization using anti-Metadherin antibody with DAB staining.
Application
Figure 3: Flow cytometric analysis of Hela cells using anti-Metadherin mAb (green) and negative control (purple).| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human MTDH expressed in E. Coli. |
