Application
Western blot analysis of METAP2 Antibody (C-term) in 293 cell line lysates (35ug/lane). METAP2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with METAP2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Methionine Aminopeptidase 2 (METAP2) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | METAP2; MNPEP; P67EIF2; Methionine aminopeptidase 2; Initiation factor 2-associated 67 kDa glycoprotein; Peptidase M 2; p67eIF2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MNPEP P67EIF2 |
|---|---|
| Alternative Names | METAP2 MNPEP P67EIF2 Methionine aminopeptidase 2 Initiation factor 2-associated 67 kDa glycoprotein Peptidase M 2 p67eIF2 |
| Molecular Weight(MW) | 52892 Da |
| Function | Removes the N-terminal methionine from nascent proteins. The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo |
| Tissue Specificity | This METAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445~475 amino acids from the C-terminal region of human METAP2. |
| Cellular Localization | Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes |
Application
Western blot analysis of METAP2 Antibody (C-term) in 293 cell line lysates (35ug/lane). METAP2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with METAP2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
