Application
MLF1IP Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MLF1IP antibody detected the MLF1IP protein (arrow).| Product Name | MLF1IP Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLF1IP; CENPU; ICEN24; KLIP1; PBIP1; Centromere protein U; Interphase centromere complex protein 24; KSHV latent nuclear antigen-interacting protein 1; MLF1-interacting protein; Polo-box-interacting protein 1; centromere protein of 50 kDa |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CENPU ICEN24 KLIP1 PBIP1 |
|---|---|
| Alternative Names | MLF1IP CENPU ICEN24 KLIP1 PBIP1 Centromere protein U Interphase centromere complex protein 24 KSHV latent nuclear antigen-interacting protein 1 MLF1-interacting protein Polo-box-interacting protein 1 centromere protein of 50 kDa |
| Molecular Weight(MW) | 47522 Da |
| Function | Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression |
| Tissue Specificity | Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells Expressed at high levels in glioblastoma cell lines |
| Cellular Localization | Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Note=Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates |
| Entrez Gene | 79682 |
|---|---|
| Nucleotide Accession | NP_078905.2 |
Application
MLF1IP Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MLF1IP antibody detected the MLF1IP protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
