Application
Western blot analysis of MLK3 (arrow) using rabbit polyclonal MLK3 Antibody (Center) (Cat# AP7921c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MLK3 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MLK3 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | MLK3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP3K11; MLK3; PTK1; SPRK; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MLK3 PTK1 SPRK |
|---|---|
| Alternative Names | MAP3K11 MLK3 PTK1 SPRK Mitogen-activated protein kinase kinase kinase 11 Mixed lineage kinase 3 Src-homology 3 domain-containing proline-rich kinase |
| Molecular Weight(MW) | 92688 Da |
| Function | Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7 Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle |
| Tissue Specificity | Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Note=Location is cell cycle dependent |
| Entrez Gene | 4296 |
|---|
Application
Western blot analysis of MLK3 (arrow) using rabbit polyclonal MLK3 Antibody (Center) (Cat# AP7921c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MLK3 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MLK3 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
