Application
A. Western blot analysis on A431 cell lysate using anti-MLK-3 RabMAb (Cat. #AJ1490a).Application
B. Immunofluorescent staining of HeLa cells using anti-MLK3 RabMAb (Cat. #AJ1490a).| Product Name | MLK-3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP3K11, MLK3, PTK1, SPRK, Mitogen-activated protein kinase kinase kinase 11, Mixed lineage kinase 3;Src-homology 3 domain-containing proline-rich kinase |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1460Y |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:25000~50000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MLK3 PTK1 SPRK |
|---|---|
| Alternative Names | MAP3K11 MLK3 PTK1 SPRK Mitogen-activated protein kinase kinase kinase 11 Mixed lineage kinase 3 Src-homology 3 domain-containing proline-rich kinase |
| Molecular Weight(MW) | 92688 Da |
| Function | Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7 Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle |
| Tissue Specificity | Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Note=Location is cell cycle dependent |
| Entrez Gene | 4296 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-MLK-3 RabMAb (Cat. #AJ1490a).
Application
B. Immunofluorescent staining of HeLa cells using anti-MLK3 RabMAb (Cat. #AJ1490a).| Application Notes | WB:1:25000~50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
