Application
Western blot analysis of hMLP-E152 in K562 cell line lysates (35ug/lane). MLP (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | MLP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MARCKSL1; MLP; MRP; MARCKS-related protein; MARCKS-like protein 1; Macrophage myristoylated alanine-rich C kinase substrate |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MLP MRP |
|---|---|
| Alternative Names | MARCKSL1 MLP MRP MARCKS-related protein MARCKS-like protein 1 Macrophage myristoylated alanine-rich C kinase substrate |
| Molecular Weight(MW) | 19529 Da |
| Function | Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration. When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration. May also affect cancer cell migration. May be involved in coupling the protein kinase C and calmodulin signal transduction systems (By similarity) |
| Tissue Specificity | This MLP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MLP. |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane (By similarity). Note=Associates with the membrane via the insertion of the N-terminal N-myristoyl chain and the partial insertion of the effector domain. Association of the effector domain with membranes may be regulated by Ca(2+)/calmodulin (By similarity) In prostate cancers, detected in the cytoplasm and associated with apical or basal membranes (By similarity) |
| Entrez Gene | 65108 |
|---|
Application
Western blot analysis of hMLP-E152 in K562 cell line lysates (35ug/lane). MLP (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
