Application
MMP14 Monoclonal Antibody (C-term) (Cat. #AM1832a) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow).(8ug/ml)Application
Confocal immunofluorescent analysis of MMP14 Antibody (C-term) (Cat#AM1832a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | MMP14 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MMP14; Matrix metalloproteinase-14; MMP-X1; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 133CT15.10.5.1 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MMP14 Matrix metalloproteinase-14 MMP-X1 Membrane-type matrix metalloproteinase 1 Membrane-type-1 matrix metalloproteinase |
|---|---|
| Molecular Weight(MW) | 65894 Da |
| Function | Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15 |
| Tissue Specificity | Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Potential). Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm |
| Entrez Gene | 4323 |
|---|---|
| Nucleotide Accession | NP_004986.1 |
Application
MMP14 Monoclonal Antibody (C-term) (Cat. #AM1832a) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow).(8ug/ml)
Application
Confocal immunofluorescent analysis of MMP14 Antibody (C-term) (Cat#AM1832a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
