Application
Western blot analysis of MMP14 Antibody (C-term) (Cat. #AM1832b) in A2058 cell line lysates (35ug/lane).MMP14 (arrow) was detected using the purified Mab.(1:1000)Application
MMP14 Antibody (C-term)(Ascites) (Cat. #AM1832b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MMP14 Antibody (C-term)(Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MMP14 Antibody (C-term)(Ascites) (Cat. #AM1832b) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Product Name | MMP14 Antibody (C-term) (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MMP14; Matrix metalloproteinase-14; MMP-X1; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 133CT15.10.5.1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:1000~16000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MMP14 Matrix metalloproteinase-14 MMP-X1 Membrane-type matrix metalloproteinase 1 Membrane-type-1 matrix metalloproteinase |
|---|---|
| Molecular Weight(MW) | 65894 Da |
| Function | Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15 |
| Tissue Specificity | Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Potential). Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm |
| Entrez Gene | 4323 |
|---|
Application
Western blot analysis of MMP14 Antibody (C-term) (Cat. #AM1832b) in A2058 cell line lysates (35ug/lane).MMP14 (arrow) was detected using the purified Mab.(1:1000)
Application
MMP14 Antibody (C-term)(Ascites) (Cat. #AM1832b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MMP14 Antibody (C-term)(Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MMP14 Antibody (C-term)(Ascites) (Cat. #AM1832b) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Application Notes | WB:1:1000~16000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
