Application
Mouse Itk Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Itk antibody detected the Itk protein (arrow).Application
Mouse Itk Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Itk antibody detected the Itk protein (arrow).| Product Name | Itk Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Itk; Emt; Tlk; Tsk; Tyrosine-protein kinase ITK/TSK; IL-2-inducible T-cell kinase; Kinase EMT; Kinase TLK; T-cell-specific kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Emt Tlk Tsk |
|---|---|
| Alternative Names | Itk Emt Tlk Tsk Tyrosine-protein kinase ITK/TSK IL-2-inducible T-cell kinase Kinase EMT Kinase TLK T-cell-specific kinase |
| Molecular Weight(MW) | 72292 Da |
| Function | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation |
| Tissue Specificity | Is detected in the thymus, lymph node and very faintly in the spleen, but is not detected in the liver, lung, kidney, heart, brain, intestine or testis. Expressed in T- lymphocytes and mast cells. It may also be expressed in natural killer cells |
| Cellular Localization | Cytoplasm. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation (By similarity) |
| Entrez Gene | 16428 |
|---|---|
| Nucleotide Accession | NP_034713.2 |
Application
Mouse Itk Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Itk antibody detected the Itk protein (arrow).
Application
Mouse Itk Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Itk antibody detected the Itk protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
