| Product Name | ITK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2F12] to ITKITK is a T cell signaling protein. The Tec family of non receptor tyrosine kinases is composed of six proteins designated Tec, ITK (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini. Tik associates with CD28 and becomes activated subsequent to CD28 ligation. |
| Immunogen | Recombinant full length protein (Mouse). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2F12 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| Immunoprecipitation (with protein G): use at a concentration of 2μg / mg of lysate. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of ~ 72 kDa, which corresponds to the predicted molecular weight of ITK.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Emt Tcsk Tsk |
|---|---|
| Target | ITK |
| Cellular Localization | Plasma membrane |
| Light Chain Type | kappa |
| Positive Control | Jurkat cells |
|---|---|
| Application Notes | Immunoprecipitation (with protein G): use at a concentration of 2μg / mg of lysate. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of ~ 72 kDa, which corresponds to the predicted molecular weight of ITK.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
