Application
Mouse Yes1 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the Yes1 antibody detected the Yes1 protein (arrow).Application
Western blot analysis of Yes1 (arrow) using rabbit polyclonal Mouse Yes1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Yes1 gene.| Product Name | Yes1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Yes |
|---|---|
| Alternative Names | Yes1 Yes Tyrosine-protein kinase Yes Proto-oncogene c-Yes p61-Yes |
| Molecular Weight(MW) | 60630 Da |
| Function | Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity) |
| Tissue Specificity | This Mouse Yes1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of Mouse Yes1. |
| Cellular Localization | Cell membrane (By similarity). Cytoplasm, cytoskeleton, centrosome (By similarity). Cytoplasm, cytosol (By similarity). Note=Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway (By similarity) |
| Entrez Gene | 22612 |
|---|---|
| Nucleotide Accession | NP_033561.1 |
Application
Mouse Yes1 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the Yes1 antibody detected the Yes1 protein (arrow).
Application
Western blot analysis of Yes1 (arrow) using rabbit polyclonal Mouse Yes1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Yes1 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
