WB
Western blot analysis of Yes1 on different lysates with Rabbit anti-Yes1 antibody at 1/1,000 dilution.Lysates/proteins at 20 µg/Lane.Exposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody t 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Yes1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Yes1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Yes1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Yes1 aa 480 to the C-terminus. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | YES1 |
|---|---|
| Gene Synonyms | Yes c-yes HsT441 P61-YES |
| Gene Full Name | YES proto-oncogene 1, Src family tyrosine kinase |
| Gene Summary | This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008] |
| Alternative Names | c Yes antibody Cellular Yes 1 protein antibody Cellular Yes1 protein antibody HsT441 antibody P61 YES antibody p61-Yes antibody Proto oncogene tyrosine protein kinase YES antibody Proto-oncogene c-Yes antibody Tyrosine-protein kinase Yes antibody v yes 1 Yamaguchi sarcoma viral oncogene homolog 1 antibody c Yes antibody Cellular Yes 1 protein antibody Cellular Yes1 protein antibody HsT441 antibody P61 YES antibody p61-Yes antibody Proto oncogene tyrosine protein kinase YES antibody Proto-oncogene c-Yes antibody Tyrosine-protein kinase Yes antibody v yes 1 Yamaguchi sarcoma viral oncogene homolog 1 antibody Viral oncogene yes 1 homolog 1 antibody Viral oncogene yes1 homolog 1 antibody Yamaguchi sarcoma oncogene antibody Yes 1 antibody Yes antibody YES proto oncogene 1 Src family tyrosine kinase antibody YES_HUMAN antibody YES1 antibody YES1 proto oncogene Src family tyrosine kinase antibody |
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | Cytoplasm > cytosol. |
WB
Western blot analysis of Yes1 on different lysates with Rabbit anti-Yes1 antibody at 1/1,000 dilution.Lysates/proteins at 20 µg/Lane.Exposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody t 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Yes1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Yes1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
