Application
Figure 1: Western blot analysis using anti-MSH2 monoclonal antibody against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using anti-MSH2 antibody with DAB staining.Application
Figure 3: Confocal immunofluorescence analysis of Hela cells using anti-MSH2 monoclonal antioby (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Product Name | MSH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1B3=3A2B8C |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500 - 2,000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | FCC1 COCA1 HNPCC LCFS2 |
|---|---|
| Molecular Weight(MW) | 104743 Da |
| Function | Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Nucleus (Potential). |
| Entrez Gene | 4436 |
|---|---|
| Nucleotide Accession | NP_000242.1 |
Application
Figure 1: Western blot analysis using anti-MSH2 monoclonal antibody against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using anti-MSH2 antibody with DAB staining.
Application
Figure 3: Confocal immunofluorescence analysis of Hela cells using anti-MSH2 monoclonal antioby (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Application Notes | WB:1:500 - 2,000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human MSH2 expressed in E. Coli. |
