Application
Western blot analysis of anti-MSH3 Antibody (Center) in Ramos cell line lysates (35ug/lane). MSH3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human kidney tissue reacted with MSH3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
MSH3 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MSH3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MSH3; DUC1; DUG; DNA mismatch repair protein Msh3; Divergent upstream protein; Mismatch repair protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DUC1 DUG |
|---|---|
| Alternative Names | MSH3 DUC1 DUG DNA mismatch repair protein Msh3 Divergent upstream protein Mismatch repair protein 1 |
| Molecular Weight(MW) | 127412 Da |
| Function | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion- deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis |
| Tissue Specificity | This MSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 466~495 amino acids from the Center region of human MSH3. |
| Entrez Gene | 4437 |
|---|
Application
Western blot analysis of anti-MSH3 Antibody (Center) in Ramos cell line lysates (35ug/lane). MSH3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human kidney tissue reacted with MSH3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
MSH3 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
